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Last Updated: August 14, 2024

Claims for Patent: 5,712,155

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Summary for Patent: 5,712,155

Title:	DNA encoding tumor necrosis factor-.alpha. and -.beta. receptors
Abstract:	Tumor necrosis factor receptor DNAs and expression vectors encoding TNF receptors, and processes for producing TNF receptors as products of recombinant cell culture, are disclosed.
Inventor(s):	Smith; Craig A. (Seattle, WA), Goodwin; Raymond G. (Seattle, WA), Beckmann; M. Patricia (Poulsbo, WA)
Assignee:	Immunex Corporation (Seattle, WA)
Application Number:	08/346,555
Patent Claims:	1. An isolated DNA sequence selected from the group consisting of: (a) a DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of amino acids 1 to X of FIG. 2A and amino acids 1 to 233 of FIG. 3A, wherein X is an amino acid from 163 to 235; and (b) a DNA sequence capable of hybridization to the complement of the DNA sequence of (a) under moderately stringent conditions (50.degree. C., 2.times. SSC) and which encodes a polypeptide that is capable of binding to TNF and which is at least 88% identical to a polypeptide encoded by the DNA of (a). 2. An isolated DNA sequence selected from the group consisting off (a) a DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of amino acids 1 to X of FIG. 2A and amino acids 1 to 233 of FIG. 3A, wherein X is an amino acid from 163 to 235; and (b) a DNA sequence capable of hybridization to the complement of the DNA sequence of (a) under moderately stringent conditions (50.degree. C., 2.times. SSC) and which encodes TNF-R protein that is capable of binding greater than 0.1 nmoles TNF per nmole TNF-R and which is at least 88% identical to a polypeptide encoded by the DNA of (a). 3. An isolated DNA sequence selected from the group consisting of: (a) a DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of amino acids 1 to X of FIG. 2A and amino acids 1 to 233 of FIG. 3A, wherein X is an amino acid from 163 to 235; and (b) a DNA sequence capable of hybridization to the complement of the DNA sequence of (a) under moderately stringent conditions (50.degree. C., 2.times. SSC) and which encodes TNF-R protein that is capable of binding greater than 0.5 nmoles TNF per nmole TNF-R and which is at least 88% identical to a polypeptide encoded by the DNA of (a). 4. A recombinant expression vector comprising the DNA sequence according to claim 1. 5. A recombinant expression vector comprising the DNA sequence according to claim 2. 6. A recombinant expression vector comprising the DNA sequence according to claim 3. 7. A host cell transformed or transfected with the vector according to claim 4. 8. A host cell transformed or transfected with the vector according to claim 5. 9. A host cell transformed or transfected with the vector according to claim 6. 10. An isolated DNA sequence selected from the group consisting of: (a) a DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of amino acids 1 to X of FIG. 2A and amino adds 1 to 233 of FIG. 3A, wherein X is an amino acid from 163 to 235; and (b) a DNA sequence that encodes a polypeptide identical to the polypeptide encoded by the DNA of (a) except for modification(s) to the amino acid sequence selected from the group consisting of: (i) inactivated N-linked glycosylation sites; (ii) altered KEX2 protease cleavage sites; (iii) conservative amino acid substitutions; (iv) substitution or deletion of cysteine residues; and (iv) combinations of modifications (i)-(iv); wherein such polypeptide is capable of binding TNF. 11. An isolated DNA sequence selected from the group consisting of: (a) a DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of amino acids 1 to X of FIG. 2A and amino acids 1 to 233 of FIG. 3A, wherein X is an amino acid from 163 to 235; and (b) a DNA sequence that encodes a polypeptide identical to the polypeptide encoded by the DNA of (a) except for modification(s) to the amino acid sequence selected from the group consisting of: (i) inactivated N-linked glycosylation sites; (ii) altered KEX2 protease cleavage sites; (ii) conservative amino acid substitutions; (iv) substitution or deletion of cysteine residues; and (v) combinations of modifications (i)-(iv); which encoded polypeptide is capable of binding greater than 0.1 moles TNF per nmole of such polypeptide. 12. An isolated DNA sequence selected from the group consisting of: (a) a DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of amino acids 1 to X of FIG. 2A and amino acids 1 to 233 of FIG. 3A, wherein X is an amino acid from 163 to 235; and (b) a DNA sequence that encodes a polypeptide identical to the polypeptide encoded by the DNA of (a) except for modification(s) to the amino acid sequence selected from the group consisting of: (i) inactivated N-linked glycosylation sites; (ii) altered KEX2 protease cleavage sites; (ii) conservative amino acid substitutions; (iv) substitution or deletion of cysteine residues; and (v) combinations of modifications (i)-(iv); which encoded polypeptide is capable of binding greater than 0.5 moles TNF per nmole of such polypeptide. 13. A recombinant expression vector comprising the DNA according to any of claims 10, 11 or 12. 14. A host cell transformed or transfected with the vector according to claim 13. 15. A DNA sequence that encodes a polypeptide having the amino acid sequence selected from the group consisting of (a) amino acids 1-235 of FIG. 2A; and (b) a DNA sequence capable of hybridization to the DNA sequence of (a) under moderately stringent conditions (50.degree. C., 2.times. SSC) and which encodes a polypeptide that is capable of binding to TNF and which is at least 88% identical to a polypeptide encoded by the DNA of (a). 16. A recombinant expression vector comprising the DNA sequence according to claim 15. 17. A host cell transformed or transfected with the vector according to claim 16.

Details for Patent 5,712,155

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Immunex Corporation	ENBREL	etanercept	For Injection	103795	November 02, 1998	⤷ Sign Up	2009-09-05
Immunex Corporation	ENBREL	etanercept	For Injection	103795	May 27, 1999	⤷ Sign Up	2009-09-05
Immunex Corporation	ENBREL	etanercept	Injection	103795	September 27, 2004	⤷ Sign Up	2009-09-05
Immunex Corporation	ENBREL	etanercept	Injection	103795	February 01, 2007	⤷ Sign Up	2009-09-05
Immunex Corporation	ENBREL MINI	etanercept	Injection	103795	September 14, 2017	⤷ Sign Up	2009-09-05
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

International Patent Family for US Patent 5,712,155

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Country	Patent Number	Estimated Expiration
South Africa	907072	⤷ Sign Up
World Intellectual Property Organization (WIPO)	9406476	⤷ Sign Up
World Intellectual Property Organization (WIPO)	9319777	⤷ Sign Up
World Intellectual Property Organization (WIPO)	9103553	⤷ Sign Up
United States of America	RE36755	⤷ Sign Up
>Country	>Patent Number	>Estimated Expiration

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