Claims for Patent: 5,236,838

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Summary for Patent: 5,236,838

Title:	Enzymatically active recombinant glucocerebrosidase
Abstract:	Recombinant enzymatically active glucocerebrosidase is produced by a eukaryotic cell. Also, a cell includes nucleic acid encoding enzymatically active glucocerebrosidase; also a eukaryotic organism contains such a cell. Also, a method for producing enzymatically active glucocerebrosidase includes steps of introducing glucocerebrosidase-encoding nucleic acid into a eukaryotic cell, causing the cell to express glucocerebrosidase, and purifying the glucocerebrosidase from the cell.
Inventor(s):	Rasmussen; James (Boston, MA), Barsomian; Gary (Georgetown, MA), Bergh; Michel (Belmont, MA)
Assignee:	Genzyme Corporation (Cambridge, MA)
Application Number:	07/748,283
Patent Claims:	1. A method for producing enzymatically active glucocerebrosidase comprising the steps of: introducing nucleic acid encoding human glucocerebrosidase into a CHO cell; causing said cell to express and secrete said glucocerebrosidase into a culture medium; and purifying said glucocerebrosidase from said culture medium. 2. The method of claim 1 wherein the pH of said culture medium is between about pH 6.5 and pH 7.2. 3. The method of claim 2 wherein the pH of said culture medium is between about pH 6.6 and pH 6.8. 4. The method of claim 1 wherein said culture medium contains O.sub.2 in an amount below about 50% saturation and sufficient to maintain the cells. 5. The method of claim 1 wherein said culture medium contains O.sub.2 in an amount between about 20% saturation and about 30% saturation. 6. A method for producing enzymatically active glucocerebrosidase comprising the steps of: introducing a plasmid containing nucleic acid encoding glucocerebrosidase into a CHO cell, said plasmid being selected from the group consisting of pGB20, pGB37 and pGB42; causing said cell to express and secrete said glucocerebrosidase into a culture medium; and purifying said glucocerebrosidase from said culture medium.

Details for Patent 5,236,838

Glossary

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Genzyme Corporation	CEREZYME	imiglucerase	For Injection	020367	May 23, 1994	⤷ Sign Up	2010-08-17
Genzyme Corporation	CEREZYME	imiglucerase	For Injection	020367	September 22, 1999	⤷ Sign Up	2010-08-17
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

International Patent Family for US Patent 5,236,838

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Country	Patent Number	Estimated Expiration
Austria	E138686	⤷ Sign Up
Canada	2006709	⤷ Sign Up
Canada	2339888	⤷ Sign Up
Germany	68926569	⤷ Sign Up
>Country	>Patent Number	>Estimated Expiration

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