Claims for Patent: 5,236,838
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Summary for Patent: 5,236,838
Title: | Enzymatically active recombinant glucocerebrosidase |
Abstract: | Recombinant enzymatically active glucocerebrosidase is produced by a eukaryotic cell. Also, a cell includes nucleic acid encoding enzymatically active glucocerebrosidase; also a eukaryotic organism contains such a cell. Also, a method for producing enzymatically active glucocerebrosidase includes steps of introducing glucocerebrosidase-encoding nucleic acid into a eukaryotic cell, causing the cell to express glucocerebrosidase, and purifying the glucocerebrosidase from the cell. |
Inventor(s): | Rasmussen; James (Boston, MA), Barsomian; Gary (Georgetown, MA), Bergh; Michel (Belmont, MA) |
Assignee: | Genzyme Corporation (Cambridge, MA) |
Application Number: | 07/748,283 |
Patent Claims: | 1. A method for producing enzymatically active glucocerebrosidase comprising the steps of:
introducing nucleic acid encoding human glucocerebrosidase into a CHO cell; causing said cell to express and secrete said glucocerebrosidase into a culture medium; and purifying said glucocerebrosidase from said culture medium. 2. The method of claim 1 wherein the pH of said culture medium is between about pH 6.5 and pH 7.2. 3. The method of claim 2 wherein the pH of said culture medium is between about pH 6.6 and pH 6.8. 4. The method of claim 1 wherein said culture medium contains O.sub.2 in an amount below about 50% saturation and sufficient to maintain the cells. 5. The method of claim 1 wherein said culture medium contains O.sub.2 in an amount between about 20% saturation and about 30% saturation. 6. A method for producing enzymatically active glucocerebrosidase comprising the steps of: introducing a plasmid containing nucleic acid encoding glucocerebrosidase into a CHO cell, said plasmid being selected from the group consisting of pGB20, pGB37 and pGB42; causing said cell to express and secrete said glucocerebrosidase into a culture medium; and purifying said glucocerebrosidase from said culture medium. |
Details for Patent 5,236,838
Applicant | Tradename | Biologic Ingredient | Dosage Form | BLA | Approval Date | Patent No. | Expiredate |
---|---|---|---|---|---|---|---|
Genzyme Corporation | CEREZYME | imiglucerase | For Injection | 020367 | May 23, 1994 | ⤷ Subscribe | 2010-08-17 |
Genzyme Corporation | CEREZYME | imiglucerase | For Injection | 020367 | September 22, 1999 | ⤷ Subscribe | 2010-08-17 |
>Applicant | >Tradename | >Biologic Ingredient | >Dosage Form | >BLA | >Approval Date | >Patent No. | >Expiredate |
International Patent Family for US Patent 5,236,838
Country | Patent Number | Estimated Expiration |
---|---|---|
World Intellectual Property Organization (WIPO) | 9007573 | ⤷ Subscribe |
United States of America | 6451600 | ⤷ Subscribe |
United States of America | 5549892 | ⤷ Subscribe |
>Country | >Patent Number | >Estimated Expiration |
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