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Last Updated: November 23, 2024

Claims for Patent: 5,441,868

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Summary for Patent: 5,441,868

Title:	Production of recombinant erythropoietin
Abstract:	Disclosed are novel polypeptides possessing part or all of the primary structural conformation and one or more of the biological properties of mammalian erythropoietin (\"EPO\") which are characterized in preferred forms by being the product of procaryotic or eucaryotic host expression of an exogenous DNA sequence. Illustratively, genomic DNA, cDNA and manufactured DNA sequences coding for part or all of the sequence of amino acid residues of EPO or for analogs thereof are incorporated into autonomously replicating plasmid or viral vectors employed to transform or transfect suitable procaryotic or eucaryotic host cells such as bacteria, yeast or vertebrate cells in culture. Upon isolation from culture media or cellular lysates or fragments, products of expression of the DNA sequences display, e.g., the immunological properties and in vitro and in vivo biological activities of EPO of human or monkey species origins. Disclosed also are chemically synthesized polypeptides sharing the biochemical and immunological properties of EPO. Also disclosed are improved methods for the detection of specific single stranded polynucleotides in a heterologous cellular or viral sample prepared from, e.g., DNA present in a plasmid or viral-borne cDNA or genomic DNA \"library\".
Inventor(s):	Lin; Fu-Kuen (Thousand Oaks, CA)
Assignee:	Kirin-Amgen, Inc. (Thousand Oaks, CA)
Application Number:	07/113,179
Patent Claims:	1. A process for the production of a glycosylated erythropoietin polypeptide having the in vivo biological property of causing bone marrow cells to increase production of reticulocytes and red blood cells comprising the steps of: (a) growing, under suitable nutrient conditions, mammalian host cells transformed or transfected with an isolated DNA sequence encoding human erythropoietin; and (b) isolating said glycosylated erythropoietin polypeptide therefrom. 2. The process according to claim 1 wherein said host cells are CHO cells. 3. The process according to claim 1 wherein said host cells are COS cells. 4. The process according to claim 1 wherein said DNA is cDNA. 5. The process according to claim 1 wherein said DNA is genomic DNA.

Details for Patent 5,441,868

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Applicant	Tradename	Biologic Ingredient	Dosage Form	BLA	Approval Date	Patent No.	Expiredate
Amgen Inc.	EPOGEN/PROCRIT	epoetin alfa	Injection	103234	June 01, 1989	⤷ Sign Up	2012-08-15
Amgen Inc.	EPOGEN/PROCRIT	epoetin alfa	Injection	103234		⤷ Sign Up	2012-08-15
Amgen Inc.	PROCRIT	epoetin alfa	Injection	103234		⤷ Sign Up	2012-08-15
>Applicant	>Tradename	>Biologic Ingredient	>Dosage Form	>BLA	>Approval Date	>Patent No.	>Expiredate

International Patent Family for US Patent 5,441,868

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Country	Patent Number	Estimated Expiration
Austria	E54940	⤷ Sign Up
Australia	1007495	⤷ Sign Up
Australia	2042192	⤷ Sign Up
Australia	3612597	⤷ Sign Up
Australia	3746785	⤷ Sign Up
Australia	5272293	⤷ Sign Up
Australia	5750490	⤷ Sign Up
>Country	>Patent Number	>Estimated Expiration

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