Claims for Patent: 6,974,861

Summary for Patent: 6,974,861

Title:	Pharmaceutical compositions and methods using natriuretic peptides
Abstract:	The cDNA sequence encoding porcine brain natriuretic peptide and related genes encoding canine and human peptides with natriuretic activity are disclosed. The gene is shown to make accessible the DNAs encoding analogous natriuretic peptides in other vertebrate species. The genes encoding these NPs can be used to effect modifications of the sequence to produce alternate forms of the NPs and to provide practical amounts of these proteins. The NPs of the invention can also be synthesized chemically. The invention peptides have the formula: wherein R.sup.1 is selected from the group consisting of: or a 10- to 109-amino acid sequence shown as the native upstream sequence for porcine, canine or human BNP in FIG. 8, or a composite thereof; R.sup.2 is (OH), NH.sub.2, or NR'R" wherein R' and R" are independently lower alkyl (1-4C) or is or the amides (NH.sub.2 or NR'R") thereof, with the proviso that if formula (1) is and R.sup.1 is Asp-Ser-Gly-, R.sup.2 cannot be Asn-Val-Leu-Arg-Arg-Tyr. The peptides of the invention can be formulated into pharmaceutical compositions and used to treat conditions associated with high extracellular fluid levels, especially congestive heart failure.
Inventor(s):	Seilhamer; J. Jeffrey (Milpitas, CA), Lewicki; John (San Jose, CA), Scarborough; Robert M. (Hayward, CA), Porter; J. Gordon (Newark, CA)
Assignee:	Scios, Inc. (Sunnyvale, CA)
Application Number:	10/402,021
Patent Claims:	1. A pharmaceutical composition which comprises an effective amount of the peptide Ser-Pro-Lys-Met-Val-Gln-Gly-Ser-Gly-Cys-Phe-Gly-Arg-Lys-Met-Asp-Arg-Ile-Se r-Ser-Ser-Ser-Gly-Leu-Gly-Cis-Lys-Val-Leu-Arg-Arg-His (SEQ ID NO:49) or the amide thereof in admixture with a suitable pharmaceutical excipient. 2. A method to induce natriuresis, diuresis and/or vasodilation in a subject in need of such treatment which method comprises administering to said subject an effective amount of the peptide Ser-Pro-Lys-Met-Val-Gln-Gly-Ser-Gly-Cys-Phe-Gly-Arg-Lys-Met-Asp-Arg-Ile-Se r-Ser-Ser-Ser-Gly-Leu-Gly-Cis-Lys-Val-Leu-Arg-Arg-His (SEQ ID NO:49) or the amide thereof or a pharmaceutical composition thereof. 3. A method to treat congestive heart failure in a subject in need of such treatment which method comprises administering to said subject an effective amount of the peptide Ser-Pro-Lys-Met-Val-Gln-Gly-Ser-Gly-Cys-Phe-Gly-Arg-Lys-Met-Asp-Arg-Ile-Se r-Ser-Ser-Ser-Gly-Leu-Gly-Cis-Lys-Val-Leu-Arg-Arg-His (SEQ ID NO:49) or the amide thereof or a pharmaceutical composition thereof.