Claims for Patent: 9,421,280

Summary for Patent: 9,421,280

Title:	Real time imaging during solid organ transplant
Abstract:	A method of determining the patency of a donor organ or at least one vessel to a donor organ in a donor subject includes administering a fluorescent dye to the donor subject, applying a sufficient amount of energy to the vessel such that the fluorescent dye fluoresces, obtaining a fluorescent image of the donor organ or the vessel attached to a donor organ, and observing the image to determine if a fluorescent signal is continuous through the vessel. A continuous fluorescent signal in the donor organ or vessel indicates the respective donor organ or vessel is patent.
Inventor(s):	Mangat; Gurpreet (Markham, CA), Brzozowski; Lukasz (Toronto, CA), Croft; Richard L. (Oakville, CA)
Assignee:	Novadaq Technologies Inc. (Mississauga, CA)
Application Number:	14/177,045
Patent Claims:	1. A method of determining the patency of at least one vessel which is attached to a donor organ in a donor subject comprising: a. administering a fluorescent dye to the donor subject; b. applying energy to the vessel such that the fluorescent dye fluoresces; c. obtaining a fluorescent image of the vessel attached to a donor organ; and d. observing the image to determine if a fluorescent signal is continuous through the vessel, wherein a continuous fluorescent signal in the vessel indicates the vessel is patent, and wherein the fluorescent dye is a tricarbocyanine dye, fluorescein isothlocyanate, rhodamine, phycoerythrin, phycocyanin, allophycocyanin, o-phthaldehyde, fluorescamine, Rose Bengal, trypan blue, fluoro-gold, or a combination thereof. 2. A method of determining the patency of a donor organ comprising: a. administering a fluorescent dye to the vessel attached to the donor organ; b. applying energy to the vessel such that the fluorescent dye fluoresces; c. obtaining a fluorescent image of the donor organ; and d. observing the image to determine if a fluorescent signal is continuous through the donor organ, wherein continuous fluorescent signal in the donor organ indicates the donor organ is patent, and wherein the fluorescent dye is a tricarbocyanine dye, fluorescein isothlocyanate, rhodamine, phycoerythrin, phycocyanin, allophycocyanin, o-phthaldehyde, fluorescamine, Rose Bengal, trypan blue, fluoro-gold, or a combination thereof. 3. The method of claim 1, wherein the donor organ is a heart, a lung, a liver, a kidney, or a pancreas. 4. The method of claim 1, wherein the fluorescent dye is administered as a bolus injection. 5. The method of claim 1, wherein the tricarbocyanine dye is indocyanine green. 6. The method of claim 1, wherein the energy is provided by a laser. 7. The method of claim 1, wherein the energy is provided by a light emitting diode. 8. The method of claim 1, wherein the energy comprises infrared light. 9. The method of claim 1, wherein the fluorescent image is obtained with a surgical microscope or an endoscope. 10. The method of claim 1, wherein the image is obtained by a charge coupled device. 11. The method of claim 1, wherein the image is obtained by a video camera. 12. The method of claim 2, wherein the donor organ is a heart, a lung, a liver, a kidney, or a pancreas. 13. The method of claim 2, wherein the fluorescent dye is administered as a bolus injection. 14. The method of claim 2, wherein the tricarbocyanine dye is indocyanine green. 15. The method of claim 2, wherein the energy is provided by a laser. 16. The method of claim 2, wherein the energy is provided by a light emitting diode. 17. The method of claim 2, wherein the energy comprises infrared light. 18. The method of claim 2, wherein the fluorescent image is obtained with a surgical microscope or an endoscope. 19. The method of claim 2, wherein the image is obtained by a charge coupled device. 20. The method of claim 2, wherein the image is obtained by a video camera.